Identification and Characterisation of microRNAs during Bovine Ovarian Follicular Development
نویسنده
چکیده
.......................................................................................... xiv Research Publications ................................................................... xviii Conference abstracts ..................................................................... xix Chapter 1: Introduction ..................................................................... 2 1.1 Folliculogenesis............................................................................................ 4 1.1.1 Pre-antral follicle development ................................................................ 6 1.1.2 Antral follicle development ..................................................................... 7 1.1.3 Ovarian follicular waves ........................................................................ 13 1.1.4 Regulation of follicular development .................................................... 18 1.1.5 Regulation of ovulation and follicle-luteal transition ............................ 44 1.2 MicroRNAs and their functional roles in ovarian follicular development 49 1.2.1 Discovery of miRNAs ............................................................................ 49 1.2.2 miRNA nomenclature ............................................................................ 50 1.2.3 miRNA biogenesis ................................................................................. 51 1.2.4 Mechanism of gene expression regulation by miRNAs ......................... 53 1.2.5 Methodological approaches for identification & discovery of miRNAs 54 1.2.6 MiRNA expression in the ovary ............................................................ 58 1.2.7 Involvement of miRNAs in ovarian function ........................................ 62 1.3 Objectives ................................................................................................... 72 Chapter 2: Materials and Methods .................................................. 74 2.1 Collection and processing of ovarian tissues ............................................. 74 2.1.1 Collection of granulosa cells (GC), follicular wall and cumulus-oocytecomplexes (COC) ............................................................................................... 75 2.2 Steroid hormone analyses in follicular fluid .............................................. 75
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